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HomeApplicationCase Study: Beadstar-mPlex® Mouse Multiplex Cytokine Detection Kit

2025-11-01

Case Study: Beadstar-mPlex® Mouse Multiplex Cytokine Detection Kit

Case Study: Beadstar-mPlex® Mouse Multiplex Cytokine Detection Kit

Overview

Beadstar-mPlex® Mouse Multiplex Cytokine Detection Kit, developed by Beadstar Biotechnology (Hainan) Co., Ltd., is a high-performance assay tool based on Flow Fluorescence Assay technology.  It enables simultaneous detection of 20 mouse cytokines, offering flexibility, efficiency, and reliability for immunological research and related studies.

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Core Technology & Working Principle

Each cytokine detection target corresponds to a magnetic fluorescent encoded microsphere with a unique fluorescence code.  The microspheres are coated with specific capture antibodies that bind precisely to the corresponding cytokines.  During detection, the coated microspheres, test samples, and PE (R-Phycoerythrin)-labeled detection antibodies are co-incubated to form a sandwich complex.  Flow cytometers then detect the fluorescent signals of the complex to obtain the expression information of various cytokines in the samples.


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Key Product Specifications

  • Detected Cytokines: 20 mouse cytokines including IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, IL-22, MCP-1 (CCL2), IFN-γ, TNF-α, GM-CSF, G-CSF, EGF, VEGF, CXCL2 (MIP-2α), CXCL5 (ENA-78), and CX3CL1 (Fractalkine).
  • Customization: Flexible combination of cytokines based on research objectives.
  • Sample Requirements: Sample sources include cell culture supernatant, serum, cerebrospinal fluid, etc.;  only 25 μL of sample is needed per test.
  • Kit Specifications: 100T (with 2 vials of standard) and 50T (with 1 vial of standard).
  • Compatible Instruments: Ordinary flow cytometers (APC/APC-Cy7 dual fluorescence channel );  domestic instruments with Luminex system optical path / Luminex 200 (customizable).
  • Washing Methods: Optional magnetic separation or centrifugation.
  • Detection Time: Approximately 3.5 hours.
  • Shelf Life: 12 months when stored at 4°C in the dark.

Cytokine Functional Classification

CategoryCytokinesCore Functions
Pro-inflammatory CytokinesIL-1α, IL-1β, IL-6, TNF-α, IL-12p70, IL-17A, IFN-γActivate inflammatory responses, promote Th1 cell differentiation, enhance cellular immunity, and mediate antiviral/antitumor effects.
Anti-inflammatory CytokinesIL-10, IL-4Inhibit immune cell activity, reduce inflammation, and promote Th2 response.
ChemokinesMcp- 1, CX 3 CL 1, CXCL 2, CXCL 5Recruit monocytes, T cells, NK cells, and neutrophils.
Hematopoietic CytokinesGM-CSF, G-CSFStimulate the production and proliferation of granulocytes and macrophages.
Growth FactorsVEGF, EGFPromote angiogenesis, epithelial cell proliferation, and wound healing.
Th Cell-related CytokinesTh1: IFN-γ, IL-12p70; Th2: IL-4, IL-5; Th17: IL-17A, IL-22; Train: IL-10Regulate cellular immunity, humoral immunity, mucosal immunity, and immune suppression.
OthersIL-2, IL-5, IL-22Promote T cell proliferation, eosinophil differentiation, and epithelial repair.